E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata

Mohammed Medhat Gharieb, Mai Taha Badawy, Gadallah Abu-ElReesh.

Abstract
Oleaginous microorganisms accumulate 20% or more of their dry weight as lipid. In the present study, 247 fungal isolates were obtained from different types of soil, marine water, and food, and screened for their ability to accumulate considerable amounts of lipid. A primary screening with Nile red staining was followed by sulfo-phosphovanillin reaction. The most potent isolate was identified employing both morphological and molecular methods as the Zygomycetous fungus, Cunninghamella echinulata AUMC14396. The lipid of this fungus was extracted, transesterified, and analyzed via GC-MS to elucidate the fatty acid (FA) composition. The fatty acid profile revealed the presence of various fatty acids with different concentrations. Of the total fatty acids, saturated FAs comprised 44.49%, while monounsaturated FAs were 19.91%, and polyunsaturated fatty acids (PUFAs) were 35.61%. The most predominant species was linoleic acid (13.79%) followed by stearic (12.78%) and oleic (12.50%) acids. The polyunsaturated fatty acid, gamma linolenic acid was present in a considerable amount (8.02%). Other important PUFAs were also found in the fatty acid profile such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. The results of this study were discussed in respect to the important applications in the context of production of pharmaceuticals and nutraceuticals.

Key words: Oleaginous fungi, lipid, fatty acids, Cunninghamella echinulata


 
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How to Cite this Article
Pubmed Style

Gharieb MM, Badawy MT, Abu-ElReesh G, . Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 259-269. doi:10.5455/egyjebb.20221107011617

Web Style

Gharieb MM, Badawy MT, Abu-ElReesh G, . Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. https://www.egyseb.net//?mno=128030 [Access: December 05, 2023]. doi:10.5455/egyjebb.20221107011617

AMA (American Medical Association) Style

Gharieb MM, Badawy MT, Abu-ElReesh G, . Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 259-269. doi:10.5455/egyjebb.20221107011617


Vancouver/ICMJE Style

Gharieb MM, Badawy MT, Abu-ElReesh G, . Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. Egypt. J. Exp. Biol. (Bot.). (2022), [cited December 05, 2023]; 18(2): 259-269. doi:10.5455/egyjebb.20221107011617


Harvard Style

Gharieb, M. M., Badawy, M. T., Abu-ElReesh, G. & (2022) Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. Egypt. J. Exp. Biol. (Bot.), 18 (2), 259-269. doi:10.5455/egyjebb.20221107011617


Turabian Style

Gharieb, Mohammed Medhat, Mai Taha Badawy, Gadallah Abu-ElReesh, and . 2022. Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 259-269. doi:10.5455/egyjebb.20221107011617


Chicago Style

Gharieb, Mohammed Medhat, Mai Taha Badawy, Gadallah Abu-ElReesh, and . "Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 259-269. doi:10.5455/egyjebb.20221107011617


MLA (The Modern Language Association) Style

Gharieb, Mohammed Medhat, Mai Taha Badawy, Gadallah Abu-ElReesh, and . "Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.2 (2022), 259-269. Print. doi:10.5455/egyjebb.20221107011617


APA (American Psychological Association) Style

Gharieb, M. M., Badawy, M. T., Abu-ElReesh, G. & (2022) Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 259-269. doi:10.5455/egyjebb.20221107011617


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