E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression

Mohamed A. El-Esawi, Enas M. El-Ballat, Ashraf Elsayed.

Abstract
Tomato (Lycopersicon esculentum L.) is an economically important food crop grown worldwide. Bacterial wilt, caused by Clavibacter michiganensis subsp. Michiganensis (Cmm), affects tomato plants, causing harmful impacts and significant losses in crop yield. Defensin priming has previously revealed great potential in the biological control of plant diseases. The present study aimed to exogenously apply defensin to tomato plants grown in hydroponic culture in order to investigate its key role in enhancing resistance to bacterial wilt. The results revealed that Cmm infection significantly reduced the relative water content, gas exchange attributes, minerals uptake and contents of photosynthetic pigments, soluble sugars and proteins in tomato plants, as compared to control plants. Moreover, Cmm infection induced the levels of total phenols, flavonoids, glycine betaine, proline, malondialdehyde, hydrogen peroxide, electrolyte leakage, ascorbate, oxidized ascorbate, reduced glutathione, antioxidant enzymes (SOD, APX, CAT) and antioxidant genes expressions in tomato plants. On the other hand, defensin treatment significantly reduced disease severity index and oxidative stress markers in Cmm-infected tomato, as compared to Cmm-infected plants. Moreover, defensin treatment significantly enhanced the relative water content, gas exchange attributes, minerals uptake and levels of photosynthetic pigments, soluble sugars, soluble proteins, total phenols, flavonoids, glycine betaine, proline, ascorbate, oxidized ascorbate, reduced glutathione, antioxidant enzymes and antioxidant genes expression in leaves of Cmm-infected tomato, as compared to Cmm-infected plants. Taken together, the present study introduced defensin treatment as a natural, safe, and eco-friendly approach to induce the biological control of bacterial wilt in tomato plants.

Key words: Tomato; soilless agriculture; defensin treatment; bacterial wilt; antioxidants; genes expression.


 
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How to Cite this Article
Pubmed Style

El-Esawi MA, El-Ballat EM, Elsayed A, . Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 75-83. doi:10.5455/egyjebb.20220326070207

Web Style

El-Esawi MA, El-Ballat EM, Elsayed A, . Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. https://www.egyseb.net//?mno=956 [Access: December 15, 2023]. doi:10.5455/egyjebb.20220326070207

AMA (American Medical Association) Style

El-Esawi MA, El-Ballat EM, Elsayed A, . Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 75-83. doi:10.5455/egyjebb.20220326070207


Vancouver/ICMJE Style

El-Esawi MA, El-Ballat EM, Elsayed A, . Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. Egypt. J. Exp. Biol. (Bot.). (2022), [cited December 15, 2023]; 18(1): 75-83. doi:10.5455/egyjebb.20220326070207


Harvard Style

El-Esawi, M. A., El-Ballat, E. M., Elsayed, A. & (2022) Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. Egypt. J. Exp. Biol. (Bot.), 18 (1), 75-83. doi:10.5455/egyjebb.20220326070207


Turabian Style

El-Esawi, Mohamed A., Enas M. El-Ballat, Ashraf Elsayed, and . 2022. Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 75-83. doi:10.5455/egyjebb.20220326070207


Chicago Style

El-Esawi, Mohamed A., Enas M. El-Ballat, Ashraf Elsayed, and . "Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 75-83. doi:10.5455/egyjebb.20220326070207


MLA (The Modern Language Association) Style

El-Esawi, Mohamed A., Enas M. El-Ballat, Ashraf Elsayed, and . "Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.1 (2022), 75-83. Print. doi:10.5455/egyjebb.20220326070207


APA (American Psychological Association) Style

El-Esawi, M. A., El-Ballat, E. M., Elsayed, A. & (2022) Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 75-83. doi:10.5455/egyjebb.20220326070207


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