E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim.

Abstract
Species characterization in the family Characeae is generally based on morphological characteristics. The dioecious charophyte green algal genus Chara L. comprises of several species, of which high levels of morphological resemblance were recorded in this study. Four Chara samples were collected from different habitats in Egypt and morphologically studied. SSR-PCR and ISSR-PCR based techniques were performed to differentiate and characterize genetic diversity in the collected species. Two SSR and eight ISSR primers were used. Genetic differentiation of two Chara species (Sp.1 and Sp.2) was assessed by analyzing two polymorphic microsatellite (SSR) loci previously identified and developed from C. canescens. Additionally, similarity matrix and cluster analysis revealed by ISSR markers suggested separation of three Chara species (Sp.1, Sp.2, and Sp.3) away from the fourth one (Sp.4). The results indicated that applied SSR and ISSR markers most likely suitable for characterization at genus level and provide an important tool for future analyses of macroalgae such as Chara species.

Key words: Characeae, Chara canescens, Morphology, Genetic characterization, ISSR, Microsatellites.


 
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Pubmed Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . Egypt. J. Exp. Biol. (Bot.). 2015; 11(2): 129-139.

Web Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . https://www.egyseb.net//?mno=201489 [Access: December 06, 2023].

AMA (American Medical Association) Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . Egypt. J. Exp. Biol. (Bot.). 2015; 11(2): 129-139.


Vancouver/ICMJE Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . Egypt. J. Exp. Biol. (Bot.). (2015), [cited December 06, 2023]; 11(2): 129-139.


Harvard Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim (2015) MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . Egypt. J. Exp. Biol. (Bot.), 11 (2), 129-139.


Turabian Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. 2015. MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 11 (2), 129-139.


Chicago Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. "MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 11 (2015), 129-139.


MLA (The Modern Language Association) Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim. "MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 11.2 (2015), 129-139. Print.


APA (American Psychological Association) Style

Hesham M. Abd El Fatah, Mohamed Ibrahim, Shafik D. Ibrahim (2015) MORPHOLOGICAL AND MOLECULAR DIVERSITY AMONG SELECTED CHARA L. SPECIES (CHARACEAE) FROM DIFFERENT HABITATS IN EGYPT . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 11 (2), 129-139.


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