E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES

Hameda El Sayed Ahmed El Sayed.

Abstract
From seedlings of Pisum sativum, L. explants of stems, leaves, roots and cotyledons were cultured on a range of growth media. Shoot organogenesis was only obtained with nodal stem and cotyledonary node explants when cultured on MS medium, with 3% sucrose, 2.0 mg L-1 BAP and 0.2 mg L-1 NAA, Callus proliferation accompanied shoot organogenesis from nodal stem explants. Successive subcultures of nodal stem callus resulted in proliferation of regenerative callus, which contained several shoot bud initials. The capacity for shoot regeneration from this callus was maintained for 5 months. High frequency rooting of these adventitious shoots was obtained on half strength MS medium with 1.5% sucrose, 0.1 mg L-1 NAA and 0.5 mg L-1 kinetin.

Key words: Pisum sativum, L., Adventitious Shoots, Organogenesis, Tissue Culture


 
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Pubmed Style

Hameda El Sayed Ahmed El Sayed. REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 143-151.

Web Style

Hameda El Sayed Ahmed El Sayed. REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. https://www.egyseb.net//?mno=186938 [Access: December 07, 2023].

AMA (American Medical Association) Style

Hameda El Sayed Ahmed El Sayed. REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 143-151.


Vancouver/ICMJE Style

Hameda El Sayed Ahmed El Sayed. REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. Egypt. J. Exp. Biol. (Bot.). (2011), [cited December 07, 2023]; 7(1): 143-151.


Harvard Style

Hameda El Sayed Ahmed El Sayed (2011) REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. Egypt. J. Exp. Biol. (Bot.), 7 (1), 143-151.


Turabian Style

Hameda El Sayed Ahmed El Sayed. 2011. REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 143-151.


Chicago Style

Hameda El Sayed Ahmed El Sayed. "REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7 (2011), 143-151.


MLA (The Modern Language Association) Style

Hameda El Sayed Ahmed El Sayed. "REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7.1 (2011), 143-151. Print.


APA (American Psychological Association) Style

Hameda El Sayed Ahmed El Sayed (2011) REGENERATION OF CALLUS AND ORGANOGENESIS FROM EXPLANTS IN PISUM SATIVUM L. USING VARIOUS BASAL MEDIUM CULTURES. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 143-151.


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The Egyptian Journal of Experimental Biology (Botany) publishes original scientific research concerning botanical sciences (Morphology, Taxonomy, Gen ... Read more.



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