E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate

Amal Fadl Abdelkader, Hala Ragab Nosair.

Abstract
Tomato plants may be exposed to different hazardous conditions such as heavy metals or wastewater mixed with water of irrigation. In this study, two-month old tomato plants were irrigated for two weeks using water, wastewater, 1mM copper acetate solution and 1mM or mercuric acetate solution. Tomato plants were then subjected to physiological and molecular analyses. Dry weight, proline, protein, total phenols, soluble sugars and photosynthetic pigments were determined. In addition, the start codon targeted (SCoT) molecular analysis using nine primers (SCoT7, SCoT9, SCoT10, SCoT11, SCoT24, SCoT28, SCoT32, SCoT35 and SCoT46) was conducted to explore differential gene expression under stress irrigation. Data revealed general decrease in dry weight except under wastewater treatment. All copper acetate, wastewater and mercuric acetate treatments triggered the production of proline and total phenols. Protein, chl a and chl b were significantly initiated by copper acetate treatment, whereas soluble sugars were superior in control along with mercuric acetate treatment. The photosynthetic pigments varied significantly under copper acetate treatment as compared to the other treatments. SCoT analysis revealed conspicuous monomorphic profile and high polymorphic pattern; the percent of polymorphism ranged from 81.8% to 100% in treated plants. Out of the nine SCoT primers only SCoT32 primer that produced no polymorphism. The 500 bp amplicon generated by SCoT24 primer was not produced in the control plants and produced in all other treatments. Thus, this 500 pb amplicon may be related to the production of antioxidative compounds e.g. phenols, proline to cope with the adverse irrigation. Molecular results of SCoT technique can be used to clarify and explain some physiological and genetic variations occurred in tomato plants under hazardous irrigation conditions.

Key words: copper acetate, mercuric acetate, phenol, proline, protein, SCoT, tomato, wastewater


 
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How to Cite this Article
Pubmed Style

Abdelkader AF, Nosair HR. Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. Egypt. J. Exp. Biol. (Bot.). 2020; 16(1): 71-81. doi:10.5455/egyjebb.20200321032352


Web Style

Abdelkader AF, Nosair HR. Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. http://www.egyseb.net//?mno=94346 [Access: June 04, 2020]. doi:10.5455/egyjebb.20200321032352


AMA (American Medical Association) Style

Abdelkader AF, Nosair HR. Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. Egypt. J. Exp. Biol. (Bot.). 2020; 16(1): 71-81. doi:10.5455/egyjebb.20200321032352



Vancouver/ICMJE Style

Abdelkader AF, Nosair HR. Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. Egypt. J. Exp. Biol. (Bot.). (2020), [cited June 04, 2020]; 16(1): 71-81. doi:10.5455/egyjebb.20200321032352



Harvard Style

Abdelkader, A. F. & Nosair, . H. R. (2020) Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. Egypt. J. Exp. Biol. (Bot.), 16 (1), 71-81. doi:10.5455/egyjebb.20200321032352



Turabian Style

Abdelkader, Amal Fadl, and Hala Ragab Nosair. 2020. Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (1), 71-81. doi:10.5455/egyjebb.20200321032352



Chicago Style

Abdelkader, Amal Fadl, and Hala Ragab Nosair. "Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 71-81. doi:10.5455/egyjebb.20200321032352



MLA (The Modern Language Association) Style

Abdelkader, Amal Fadl, and Hala Ragab Nosair. "Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.1 (2020), 71-81. Print. doi:10.5455/egyjebb.20200321032352



APA (American Psychological Association) Style

Abdelkader, A. F. & Nosair, . H. R. (2020) Physiological and SCoT molecular analyses of Tomato (Solanum lycopersicum M.) plants in response to hazardous irrigation: wastewater, copper acetate and mercuric acetate. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (1), 71-81. doi:10.5455/egyjebb.20200321032352