E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L

Ashraf Haider, Mohammed El-Essawi, Salma Abdel-Daiem.

Abstract
Brassica campestris could be used for medicinal purposes. The plant is rich in flavonoids and phenolics that have potential for pharmacological purposes. As such, enhancement of secondary metabolites in Brassica campestris plants is highly recommended. Therefore, the aim of this study is to obtain cultures of hairy roots of Brassica campestris, which has undergone a bacterial transformation using Agrobacterium rhizogenes in an attempt to enhance the production of secondary metabolites. The level of expression of root inducing gene in transformed hairy roots was estimated using quantitative real-time PCR analysis. Results revealed higher root inducing gene expression levels in the transgenic plants as compared to the non–transformed roots. Transformed hairy roots showed higher increases in the total phenolic content, as compared to non-transformed roots. This study indicated the enhanced secondary metabolites biosynthesis by hairy roots induction in Brassica campestris plants.

Key words: Brassica campestris, Agrobacterium rhizogenes, hairy root induction, phenolics


 
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How to Cite this Article
Pubmed Style

Haider A, El-Essawi M, Abdel-Daiem S, . Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. Egypt. J. Exp. Biol. (Bot.). 2021; 17(1): 55-58. doi:10.5455/egyjebb.20210626064539


Web Style

Haider A, El-Essawi M, Abdel-Daiem S, . Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. http://www.egyseb.net//?mno=90908 [Access: July 09, 2021]. doi:10.5455/egyjebb.20210626064539


AMA (American Medical Association) Style

Haider A, El-Essawi M, Abdel-Daiem S, . Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. Egypt. J. Exp. Biol. (Bot.). 2021; 17(1): 55-58. doi:10.5455/egyjebb.20210626064539



Vancouver/ICMJE Style

Haider A, El-Essawi M, Abdel-Daiem S, . Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. Egypt. J. Exp. Biol. (Bot.). (2021), [cited July 09, 2021]; 17(1): 55-58. doi:10.5455/egyjebb.20210626064539



Harvard Style

Haider, A., El-Essawi, M., Abdel-Daiem, S. & (2021) Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. Egypt. J. Exp. Biol. (Bot.), 17 (1), 55-58. doi:10.5455/egyjebb.20210626064539



Turabian Style

Haider, Ashraf, Mohammed El-Essawi, Salma Abdel-Daiem, and . 2021. Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (1), 55-58. doi:10.5455/egyjebb.20210626064539



Chicago Style

Haider, Ashraf, Mohammed El-Essawi, Salma Abdel-Daiem, and . "Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17 (2021), 55-58. doi:10.5455/egyjebb.20210626064539



MLA (The Modern Language Association) Style

Haider, Ashraf, Mohammed El-Essawi, Salma Abdel-Daiem, and . "Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17.1 (2021), 55-58. Print. doi:10.5455/egyjebb.20210626064539



APA (American Psychological Association) Style

Haider, A., El-Essawi, M., Abdel-Daiem, S. & (2021) Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (1), 55-58. doi:10.5455/egyjebb.20210626064539