E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt

Mohamed T. Shaaban, Hussein S. Salama.

Abstract
Cellulose-producing bacteria were isolated from different sources as rotten fruits, rotten vegetables and soil samples. The most efficient cellulose-producing bacteria was isolated from rotten local apple and identified as Komagataeibacter hansenii (MH1) by biochemical, morphological and 16S rRNA testes. Different media for bacterial cellulose (BC) production were tested and the most efficient one was glucose ethanol acetic acid medium (GAM medium). Optimization of nutritional and environmental conditions for bacterial cellulose production were studied. The best conditions for bacterial cellulose biosynthesis by Komagataeibacter hansenii (MH1) were 20 g/l sucrose, 10 g/l peptone, 4 ml/l acetic acid, 7ml/l ethanol at 30C, pH 3.46 for 15 days under static culture with 5.86 g/l bacterial cellulose production. The isolated strain Komagataeibacter hansenii (MH1) was found to produce cellulose on low cost raw material begasse as it can use it as carbon source with 1.71 g/l dry BC and use it also as carbon+ nitrogen source with 1.41g/l dry BC for bacterial cellulose production but the amount of BC produced was lower than that produced on modified GAM medium.

Key words: Bacterial cellulose, Komagataeibacter hansenii, rotten fruits, begasse.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed T. Shaaban
Articles by Hussein S. Salama
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

MTS, Salama HS. Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 441-451. doi:10.5455/egyjebb.20191218083859


Web Style

MTS, Salama HS. Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. http://www.egyseb.net//?mno=78541 [Access: October 30, 2020]. doi:10.5455/egyjebb.20191218083859


AMA (American Medical Association) Style

MTS, Salama HS. Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 441-451. doi:10.5455/egyjebb.20191218083859



Vancouver/ICMJE Style

MTS, Salama HS. Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. Egypt. J. Exp. Biol. (Bot.). (2019), [cited October 30, 2020]; 15(2): 441-451. doi:10.5455/egyjebb.20191218083859



Harvard Style

, M. T. S. & Salama, . H. S. (2019) Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. Egypt. J. Exp. Biol. (Bot.), 15 (2), 441-451. doi:10.5455/egyjebb.20191218083859



Turabian Style

, Mohamed T. Shaaban, and Hussein S. Salama. 2019. Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 441-451. doi:10.5455/egyjebb.20191218083859



Chicago Style

, Mohamed T. Shaaban, and Hussein S. Salama. "Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 441-451. doi:10.5455/egyjebb.20191218083859



MLA (The Modern Language Association) Style

, Mohamed T. Shaaban, and Hussein S. Salama. "Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 441-451. Print. doi:10.5455/egyjebb.20191218083859



APA (American Psychological Association) Style

, M. T. S. & Salama, . H. S. (2019) Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 441-451. doi:10.5455/egyjebb.20191218083859