E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis

Ali El-Sayed Ali Ali, Iman Amin El-kholy, Ahmed Abd El-Rhman Ismaiel, Mohammed Abd El-Razek Mohammed, Al-zahraa Ahmed Karam El-din, Yousseria Mohammed Hassan Shetaia.

Abstract
Pulmonary mycosis (PM) diagnosis usually represents a great challenge as there are no specific pathognomonic or radiological characteristics. The aim of this study was to report the prevalence of respiratory infection with fungal pathogens in patients with chronic obstructive pulmonary disease (COPD), the predisposing factors and the clinical characteristics of the infected patients. Molecular identification of the fungal pathogens and phylogenetic trees based on 18S rRNA sequences were performed to depict the relationship between the isolated strains. Out of 250 patients admitted with COPD, 93 cases (37.20%) were positive for fungal infection while 157 cases (62.8 %) were negative for this infection. The risk factors were mainly the corticosteroid use, diabetes mellitus, smoking, prolonged antibiotic usage, human immunodeficiency virus (HIV) infection and chemotherapy. Most of the fungal infections were of the filamentous type recording 70.97%, whereas Candida spp. comprised 29.03% of the causative agents. A. fumigatus and A. flavus were the most prevalent fungal pathogens (18.28% for each). Sensitivity test was carried out against isolated pathogenic yeast and moulds using different antifungals. Ketoconazole, Posaconazole and Itraconazole had the highest percentage of sensitivity against the isolated pathogens; 82.2%, 67.7% and 65.65, respectively.

Key words: Pulmonary mycosis, Chronic obstructive pulmonary disease (COPD), prevalence, risk factors, molecular identification, sensitivity tests.


 
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How to Cite this Article
Pubmed Style

Ali AEA, El-kholy IA, Ismaiel AAE, MAEM, El-din AAK, Shetaia YMH, . A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 63-73. doi:10.5455/egyjebb.20220216032804


Web Style

Ali AEA, El-kholy IA, Ismaiel AAE, MAEM, El-din AAK, Shetaia YMH, . A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. https://www.egyseb.net//?mno=72139 [Access: April 05, 2022]. doi:10.5455/egyjebb.20220216032804


AMA (American Medical Association) Style

Ali AEA, El-kholy IA, Ismaiel AAE, MAEM, El-din AAK, Shetaia YMH, . A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 63-73. doi:10.5455/egyjebb.20220216032804



Vancouver/ICMJE Style

Ali AEA, El-kholy IA, Ismaiel AAE, MAEM, El-din AAK, Shetaia YMH, . A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. Egypt. J. Exp. Biol. (Bot.). (2022), [cited April 05, 2022]; 18(1): 63-73. doi:10.5455/egyjebb.20220216032804



Harvard Style

Ali, A. E. A., El-kholy, I. A., Ismaiel, A. A. E., , M. A. E. M., El-din, A. A. K., Shetaia, Y. M. H. & (2022) A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. Egypt. J. Exp. Biol. (Bot.), 18 (1), 63-73. doi:10.5455/egyjebb.20220216032804



Turabian Style

Ali, Ali El-Sayed Ali, Iman Amin El-kholy, Ahmed Abd El-Rhman Ismaiel, Mohammed Abd El-Razek Mohammed, Al-zahraa Ahmed Karam El-din, Yousseria Mohammed Hassan Shetaia, and . 2022. A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 63-73. doi:10.5455/egyjebb.20220216032804



Chicago Style

Ali, Ali El-Sayed Ali, Iman Amin El-kholy, Ahmed Abd El-Rhman Ismaiel, Mohammed Abd El-Razek Mohammed, Al-zahraa Ahmed Karam El-din, Yousseria Mohammed Hassan Shetaia, and . "A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 63-73. doi:10.5455/egyjebb.20220216032804



MLA (The Modern Language Association) Style

Ali, Ali El-Sayed Ali, Iman Amin El-kholy, Ahmed Abd El-Rhman Ismaiel, Mohammed Abd El-Razek Mohammed, Al-zahraa Ahmed Karam El-din, Yousseria Mohammed Hassan Shetaia, and . "A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.1 (2022), 63-73. Print. doi:10.5455/egyjebb.20220216032804



APA (American Psychological Association) Style

Ali, A. E. A., El-kholy, I. A., Ismaiel, A. A. E., , M. A. E. M., El-din, A. A. K., Shetaia, Y. M. H. & (2022) A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 63-73. doi:10.5455/egyjebb.20220216032804