E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Hexavalent chromate reduction in soil microcosm using bacterial consortium

Alaa Fayez Elsayed, Einas El Shatoury, Sahar Tolba.

Abstract
Hexavalent chromium is a toxic heavy metal used in different industries and causes environmental and health problems. In this study three bacterial isolates capable of growing at high concentration of chromium were recovered from Chromium polluted sludge. Chromium reduction was optimized and the optimum pH, temperature and aeration were 7, 30 ͦ C 200 rpm respectively for all isolates. The bacterial isolates were identified by 16S rRNA gene sequence analysis as Bacillus sp., Streptomyces rochei and Pseudomonas sp.. The isolates were mixed and tested as a consortium in Cr(VI) contaminated soil microcosm and showed complete removal of Cr(VI) in soil containing 200 mg Kg-1 and 400 mg Kg-1 after 4 and 7 days of incubation respectively. To determine the possible mechanism of Cr(VI) detoxification, cells of Bacillus sp. and Streptomyces rochei were sonicated after growth on media containing chromate and partial accumulation of chromate inside the bacterial cells was detected. Scanning electron micrograph and Energy dispersive X-ray analysis (EDX) showed adhesion of chromium on cells surface. Transmission electron micrograph showed accumulation of chromium particles inside the cells. The mixed consortium could be used for the bioremediation of waste containing hexavalent chromium.

Key words: Chromium reduction; consortium; optimization; microcosm; Tannery effluent.


 
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How to Cite this Article
Pubmed Style

Elsayed AF, Shatoury EE, Tolba S, . Hexavalent chromate reduction in soil microcosm using bacterial consortium. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): -. doi:10.5455/egyjebb.20190818073117


Web Style

Elsayed AF, Shatoury EE, Tolba S, . Hexavalent chromate reduction in soil microcosm using bacterial consortium. http://www.egyseb.net/?mno=61728 [Access: September 15, 2019]. doi:10.5455/egyjebb.20190818073117


AMA (American Medical Association) Style

Elsayed AF, Shatoury EE, Tolba S, . Hexavalent chromate reduction in soil microcosm using bacterial consortium. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): -. doi:10.5455/egyjebb.20190818073117



Vancouver/ICMJE Style

Elsayed AF, Shatoury EE, Tolba S, . Hexavalent chromate reduction in soil microcosm using bacterial consortium. Egypt. J. Exp. Biol. (Bot.). (2019), [cited September 15, 2019]; 15(2): -. doi:10.5455/egyjebb.20190818073117



Harvard Style

Elsayed, A. F., Shatoury, E. E., Tolba, S. & (2019) Hexavalent chromate reduction in soil microcosm using bacterial consortium. Egypt. J. Exp. Biol. (Bot.), 15 (2), -. doi:10.5455/egyjebb.20190818073117



Turabian Style

Elsayed, Alaa Fayez, Einas El Shatoury, Sahar Tolba, and . 2019. Hexavalent chromate reduction in soil microcosm using bacterial consortium. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), -. doi:10.5455/egyjebb.20190818073117



Chicago Style

Elsayed, Alaa Fayez, Einas El Shatoury, Sahar Tolba, and . "Hexavalent chromate reduction in soil microcosm using bacterial consortium." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), -. doi:10.5455/egyjebb.20190818073117



MLA (The Modern Language Association) Style

Elsayed, Alaa Fayez, Einas El Shatoury, Sahar Tolba, and . "Hexavalent chromate reduction in soil microcosm using bacterial consortium." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), -. Print. doi:10.5455/egyjebb.20190818073117



APA (American Psychological Association) Style

Elsayed, A. F., Shatoury, E. E., Tolba, S. & (2019) Hexavalent chromate reduction in soil microcosm using bacterial consortium. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), -. doi:10.5455/egyjebb.20190818073117