E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel

Alaa M. Abou Zeid, Eman H. Abd El Zaher, Khalil M. Saad Allah, Reham U. Ahmed.

Abstract
Depletion of fossil fuel is the largest challenge facing human industrial and technological revolution in the current era. Exploration of widely distributed microorganisms for the production of renewable biofuel is the most priority for scientific research in the last decades. In this context, thirteen fungal isolates were collected from Tanta city, El-Gharbia Governorate, Egypt belonging to four genera (Aspergillus, Penicillium, Fusarium and Alternaria) were screened on minimal media for their ability to accumulate lipid in their biomass. The maximum lipid contents were 25.31% for Aspergillus versicolor and 20.15% for Aspergillus niveus. The optimization result showed that pH 4, incubation temperature 32C, sucrose at concentration of 40g/L, glutamic acid at concentration of 0.5 g/L (C/N ratio at 40:0.5 w/w) after 5 days of incubation with agitation rate of 150 rpm on orbital shaker were the most appropriate conditions for the highest lipid accumulation in the case of Aspergillus versicolor. However, in case of Aspergillus niveus, pH 4, incubation temperature at 32C, sucrose at concentration of 20 g/L, NaNO3 at concentration of 1.0 g/L (C/N ratio at 20:1.0 w/w) after 11 days of incubation with agitation rate of 150 rpm were the most appropriate for the highest lipid accumulation.

Key words: Oleaginous fungi, biofuel, Aspergillus spp, lipid optimization


 
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How to Cite this Article
Pubmed Style

Zeid AMA, Zaher EHAE, Allah KMS, Ahmed RU, . Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 243-250. doi:10.5455/egyjebb.20190709123823


Web Style

Zeid AMA, Zaher EHAE, Allah KMS, Ahmed RU, . Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . http://www.egyseb.net/?mno=56161 [Access: October 20, 2019]. doi:10.5455/egyjebb.20190709123823


AMA (American Medical Association) Style

Zeid AMA, Zaher EHAE, Allah KMS, Ahmed RU, . Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 243-250. doi:10.5455/egyjebb.20190709123823



Vancouver/ICMJE Style

Zeid AMA, Zaher EHAE, Allah KMS, Ahmed RU, . Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . Egypt. J. Exp. Biol. (Bot.). (2019), [cited October 20, 2019]; 15(2): 243-250. doi:10.5455/egyjebb.20190709123823



Harvard Style

Zeid, A. M. A., Zaher, E. H. A. E., Allah, K. M. S., Ahmed, R. U. & (2019) Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . Egypt. J. Exp. Biol. (Bot.), 15 (2), 243-250. doi:10.5455/egyjebb.20190709123823



Turabian Style

Zeid, Alaa M. Abou, Eman H. Abd El Zaher, Khalil M. Saad Allah, Reham U. Ahmed, and . 2019. Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 243-250. doi:10.5455/egyjebb.20190709123823



Chicago Style

Zeid, Alaa M. Abou, Eman H. Abd El Zaher, Khalil M. Saad Allah, Reham U. Ahmed, and . "Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 243-250. doi:10.5455/egyjebb.20190709123823



MLA (The Modern Language Association) Style

Zeid, Alaa M. Abou, Eman H. Abd El Zaher, Khalil M. Saad Allah, Reham U. Ahmed, and . "Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 243-250. Print. doi:10.5455/egyjebb.20190709123823



APA (American Psychological Association) Style

Zeid, A. M. A., Zaher, E. H. A. E., Allah, K. M. S., Ahmed, R. U. & (2019) Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 243-250. doi:10.5455/egyjebb.20190709123823