E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.)

Elhamy Mostafa El-Assiuty, Eihab Mohamed Taha, Zeinab Mohamed Fahmy, Gamal Mohamed Fahmy.

Abstract
The oospores of Perosnospora variabilis Gäum, the causal agent of downy mildew of quinoa (Chenopodium quinoa L.), were detected histologically in the perianth of quinoa seed, the pericarp, testa, perisperm, and the cotyledons of the embryo. The histological detections of oospores were confirmed by the polymerase chain reaction (PCR) using the DNA extracted from the perianth and all of the seed parts. The percentage occurrence of oospores in examined seed samples was high in the perianth (90%) followed by the seed coat (87%) whereas, the lowest percentages were found in the embryo (3%) and the perisperm (2%).

Key words: Chenopodiaceae, Downy mildew, Sexual stages, PCR.


 
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How to Cite this Article
Pubmed Style

EME, Taha EM, ZMF, Fahmy GM. Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 197-203. doi:10.5455/egyjebb.20190620115158


Web Style

EME, Taha EM, ZMF, Fahmy GM. Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). http://www.egyseb.net/?mno=53630 [Access: August 19, 2019]. doi:10.5455/egyjebb.20190620115158


AMA (American Medical Association) Style

EME, Taha EM, ZMF, Fahmy GM. Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 197-203. doi:10.5455/egyjebb.20190620115158



Vancouver/ICMJE Style

EME, Taha EM, ZMF, Fahmy GM. Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). Egypt. J. Exp. Biol. (Bot.). (2019), [cited August 19, 2019]; 15(2): 197-203. doi:10.5455/egyjebb.20190620115158



Harvard Style

, E. M. E., Taha, . E. M., , . Z. M. F. & Fahmy, . G. M. (2019) Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). Egypt. J. Exp. Biol. (Bot.), 15 (2), 197-203. doi:10.5455/egyjebb.20190620115158



Turabian Style

, Elhamy Mostafa El-Assiuty, Eihab Mohamed Taha, Zeinab Mohamed Fahmy, and Gamal Mohamed Fahmy. 2019. Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 197-203. doi:10.5455/egyjebb.20190620115158



Chicago Style

, Elhamy Mostafa El-Assiuty, Eihab Mohamed Taha, Zeinab Mohamed Fahmy, and Gamal Mohamed Fahmy. "Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.)." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 197-203. doi:10.5455/egyjebb.20190620115158



MLA (The Modern Language Association) Style

, Elhamy Mostafa El-Assiuty, Eihab Mohamed Taha, Zeinab Mohamed Fahmy, and Gamal Mohamed Fahmy. "Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.)." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 197-203. Print. doi:10.5455/egyjebb.20190620115158



APA (American Psychological Association) Style

, E. M. E., Taha, . E. M., , . Z. M. F. & Fahmy, . G. M. (2019) Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.). THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 197-203. doi:10.5455/egyjebb.20190620115158