E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus

Sabha Ahmed Al-Sabbagh, Omima Abd El-latif Eissa, Maged Hosni Elsayed Sallam.

Abstract
Different endopthytic fungi isolated from Catheranthus roseus were tested for their ability to produce Taxol. Taxol extracted from fermentation culture of the isolated fungus Cladosporium‎ cladosporioides was identified by UV, TLC, mass spectroscopy and HPLC. The isolated strain was identified as Cladosporium‎ cladosporioides based on morphological characteristics and the analysis of the 18S rDNA sequence. HPLC quantification of fungal Taxol showed that Cladosporium‎ cladosporioides was capable of producing 700µg/l of Taxol on potato dextrose broth. The optimum culture conditions for Taxol production by the isolated Cladosporium‎ cladosporioides were tested on M1D medium after 10 days of incubation at 25 °C, pH 5.5 in the presence of sucrose and ammonium tartrate as carbon and nitrogen sources respectively. Also different concentrations of benzoic acid, 4-hydroxy benzoic acid, vanadyl sulphate, serinol and salicylic acid were evaluated for their inducing activity on Taxol production by the isolated endophytic fungus. This study offers important information and a new source for the production of the important anticancer drug Taxol by endophytic fungus fermentation. The antitumor activity of fungal Taxol was tested against human tumor cell line HepG-2 (liver carcinoma) using in vitro clonogenic assays.

Key words: Taxol production, endophytic fungus, Cladosporium‎ cladosporioides, Catheranthus roseus


 
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How to Cite this Article
Pubmed Style

Al-Sabbagh SA, , , . Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. Egypt. J. Exp. Biol. (Bot.). 2019; 15(1): 13-28. doi:10.5455/egyjebb.20181122122145


Web Style

Al-Sabbagh SA, , , . Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. http://www.egyseb.net/?mno=18422 [Access: April 22, 2019]. doi:10.5455/egyjebb.20181122122145


AMA (American Medical Association) Style

Al-Sabbagh SA, , , . Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. Egypt. J. Exp. Biol. (Bot.). 2019; 15(1): 13-28. doi:10.5455/egyjebb.20181122122145



Vancouver/ICMJE Style

Al-Sabbagh SA, , , . Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. Egypt. J. Exp. Biol. (Bot.). (2019), [cited April 22, 2019]; 15(1): 13-28. doi:10.5455/egyjebb.20181122122145



Harvard Style

Al-Sabbagh, S. . A. ., , & (2019) Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. Egypt. J. Exp. Biol. (Bot.), 15 (1), 13-28. doi:10.5455/egyjebb.20181122122145



Turabian Style

Al-Sabbagh, Sabha Ahmed , Omima Abd El-latif Eissa, Maged Hosni Elsayed Sallam, and . 2019. Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (1), 13-28. doi:10.5455/egyjebb.20181122122145



Chicago Style

Al-Sabbagh, Sabha Ahmed , Omima Abd El-latif Eissa, Maged Hosni Elsayed Sallam, and . "Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 13-28. doi:10.5455/egyjebb.20181122122145



MLA (The Modern Language Association) Style

Al-Sabbagh, Sabha Ahmed , Omima Abd El-latif Eissa, Maged Hosni Elsayed Sallam, and . "Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.1 (2019), 13-28. Print. doi:10.5455/egyjebb.20181122122145



APA (American Psychological Association) Style

Al-Sabbagh, S. . A. ., , & (2019) Taxol production by an endophytic fungus, Cladosporium‎ cladosporioides isolated from Catheranthus roseus. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (1), 13-28. doi:10.5455/egyjebb.20181122122145