E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets

Mohamed T. Shaaban, Ohood Sallam, Amal S. Abo-Senna, Amany Ahmed.

Abstract
Lactobacillus bacterial species isolated from their natural sources such as raw milk, whey, yogurts, and cheeses were identified as guided by their 16s rDNA molecular markers. Two specific phages were isolated from the identified Lactobacillus species. The isolated phages were designated as LPS1 and LPS2. The physical and chemical characteristics of both phages were studied. The results showed clear plaques with approximate diameters of 2.7 and 3.5 mm for LPS1 and LPS2, respectively. Morphological studies of the isolated phages as investigated by the transmission electron microscopy show that LPS1 and LPS2 belongs to Siphoviridae and Podoviridae, respectively. The physical properties show that these phages can tolerate UV, temperature up to 70°C and a wide range of pH degrees. The phage LPS1 show a higher tolerance towards the acidic pH in comparison to LPS2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) of structural protein from LPS1 and LPS2 phages show that the protein's molecular weight of LPS1 phage is approximately 28 kDa, but that of LPS2 phage is approximately 31.5 kDa. Finally, the chemical properties of the isolated phages indicated that genetic material of both phages is dsDNA. The genome size of both isolated phages roughly was estimated from restriction fragments and is approximately 30:35 kbp.

Key words: Lactobacillus, bacteriophage, physical and chemical proprieties.


 
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How to Cite this Article
Pubmed Style

Shaaban MT, Sallam O, Abo-Senna AS, Ahmed A, . Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 231-242. doi:10.5455/egyjebb.20201201084007


Web Style

Shaaban MT, Sallam O, Abo-Senna AS, Ahmed A, . Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. http://www.egyseb.net//?mno=17719 [Access: December 21, 2020]. doi:10.5455/egyjebb.20201201084007


AMA (American Medical Association) Style

Shaaban MT, Sallam O, Abo-Senna AS, Ahmed A, . Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 231-242. doi:10.5455/egyjebb.20201201084007



Vancouver/ICMJE Style

Shaaban MT, Sallam O, Abo-Senna AS, Ahmed A, . Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. Egypt. J. Exp. Biol. (Bot.). (2020), [cited December 21, 2020]; 16(2): 231-242. doi:10.5455/egyjebb.20201201084007



Harvard Style

Shaaban, M. T., Sallam, O., Abo-Senna, A. S., Ahmed, A. & (2020) Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. Egypt. J. Exp. Biol. (Bot.), 16 (2), 231-242. doi:10.5455/egyjebb.20201201084007



Turabian Style

Shaaban, Mohamed T., Ohood Sallam, Amal S. Abo-Senna, Amany Ahmed, and . 2020. Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 231-242. doi:10.5455/egyjebb.20201201084007



Chicago Style

Shaaban, Mohamed T., Ohood Sallam, Amal S. Abo-Senna, Amany Ahmed, and . "Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 231-242. doi:10.5455/egyjebb.20201201084007



MLA (The Modern Language Association) Style

Shaaban, Mohamed T., Ohood Sallam, Amal S. Abo-Senna, Amany Ahmed, and . "Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.2 (2020), 231-242. Print. doi:10.5455/egyjebb.20201201084007



APA (American Psychological Association) Style

Shaaban, M. T., Sallam, O., Abo-Senna, A. S., Ahmed, A. & (2020) Characterization of Lactobacillus phages isolated from dairy products handled in Egyptian markets. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 231-242. doi:10.5455/egyjebb.20201201084007