E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Optimization of L-glutaminase production by Penicillium chrysogenum

Hamed M. El-Shora, Metwally A. Metwally, Mohammed O. Zaki.

Abstract
L-glutaminase (E.C 3.5.1.2) was isolated from Penicillium chrysogenum AUMC 14100 gb, Accession No. MN219732. The enzyme was extracellular as judged from the comparison between extracellular and intracellular activities. Optimization of L-glutaminase was carried out in the light of seven parameters. These parameters were: incubation period, carbon sources, nitrogen sources, pH, temperature, inoculum size and agitation speed. The production of L-glutaminase was investigated throughout 8 days and the optimal incubation period for its production was 6 days. The best carbon and nitrogen sources were glucose and L-glutamine, respectively. The optimum concentrations of glucose and L-glutamine were 2 % w/v and 0.5 % w/v, respectively. The optimum pH and temperature for the enzyme production were 8 and 40C. The inoculum size at 100 l (107 spore/ml) and agitation speed at 200 rpm proved to be optimal parameters for the enzyme production.

Key words: L-glutaminase production, Penicillium chrysogenum


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hamed M. El-Shora
Articles by Metwally A. Metwally
Articles by Mohammed O. Zaki
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

El-Shora HM, Metwally MA, Zaki MO, . Optimization of L-glutaminase production by Penicillium chrysogenum. Egypt. J. Exp. Biol. (Bot.). 2021; 17(2): 157-165. doi:10.5455/egyjebb.20211118082536


Web Style

El-Shora HM, Metwally MA, Zaki MO, . Optimization of L-glutaminase production by Penicillium chrysogenum. http://www.egyseb.net//?mno=14021 [Access: November 23, 2021]. doi:10.5455/egyjebb.20211118082536


AMA (American Medical Association) Style

El-Shora HM, Metwally MA, Zaki MO, . Optimization of L-glutaminase production by Penicillium chrysogenum. Egypt. J. Exp. Biol. (Bot.). 2021; 17(2): 157-165. doi:10.5455/egyjebb.20211118082536



Vancouver/ICMJE Style

El-Shora HM, Metwally MA, Zaki MO, . Optimization of L-glutaminase production by Penicillium chrysogenum. Egypt. J. Exp. Biol. (Bot.). (2021), [cited November 23, 2021]; 17(2): 157-165. doi:10.5455/egyjebb.20211118082536



Harvard Style

El-Shora, H. M., Metwally, M. A., Zaki, M. O. & (2021) Optimization of L-glutaminase production by Penicillium chrysogenum. Egypt. J. Exp. Biol. (Bot.), 17 (2), 157-165. doi:10.5455/egyjebb.20211118082536



Turabian Style

El-Shora, Hamed M., Metwally A. Metwally, Mohammed O. Zaki, and . 2021. Optimization of L-glutaminase production by Penicillium chrysogenum. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (2), 157-165. doi:10.5455/egyjebb.20211118082536



Chicago Style

El-Shora, Hamed M., Metwally A. Metwally, Mohammed O. Zaki, and . "Optimization of L-glutaminase production by Penicillium chrysogenum." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17 (2021), 157-165. doi:10.5455/egyjebb.20211118082536



MLA (The Modern Language Association) Style

El-Shora, Hamed M., Metwally A. Metwally, Mohammed O. Zaki, and . "Optimization of L-glutaminase production by Penicillium chrysogenum." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17.2 (2021), 157-165. Print. doi:10.5455/egyjebb.20211118082536



APA (American Psychological Association) Style

El-Shora, H. M., Metwally, M. A., Zaki, M. O. & (2021) Optimization of L-glutaminase production by Penicillium chrysogenum. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (2), 157-165. doi:10.5455/egyjebb.20211118082536