E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.

Nessma Ahmed El Zawawy, Samira Ismail Soliman, Nada Hani Hantera.

Abstract
Down syndrome (DS) patients have a high risk of opportunistic infections from Candida species. Therefore, the purpose of this research was to assess the prevalence of Candida species in oral cavity of DS patients and investigating of grape seed extract (GSE) as a potential anticandidal agent. Our study included fifty patients with DS and fifty healthy children. Oral tongue swabs were taken from both groups and isolated Candida species were identified. The anticandidal activity of GSE against isolated Candida was studied using agar well diffusion method. Scanning electron microscope (SEM) was used to determine the major alterations in Candida after GSE treatment. GSE was subjected to GC-MS analysis and HPLC analysis. Forty six samples showed growth of Candida in DS group, where as ten samples in control group. The most common pathogenic Candida isolated from DS patients was C.tropicalis (65.2%). GSE showed significant inhibitory activity against C.tropicalis (MIC 30 mg/ml). The SEM showed complete alterations in Candida cell morphology. GC-MS chromatogram of GSE revealed 30 compounds, the major one is (9, 12-Octadecadienoic acid) which confirmed by HPLC chromatogram with a peak at RT 20.311 min. GSE could be a promising anticandidal agent and it may form a primary platform for further toxicological assessment and development of new drugs.

Key words: Down syndrome, Oral Candidal carriage, Grape seed extract, Scanning electron microscope, Gas chromatography-Mass spectroscopy.


 
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How to Cite this Article
Pubmed Style

Zawawy NAE, Soliman SI, Hantera NH. Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 169-176. doi:10.5455/egyjebb.20200825122502


Web Style

Zawawy NAE, Soliman SI, Hantera NH. Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. http://www.egyseb.net//?mno=128642 [Access: November 03, 2020]. doi:10.5455/egyjebb.20200825122502


AMA (American Medical Association) Style

Zawawy NAE, Soliman SI, Hantera NH. Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 169-176. doi:10.5455/egyjebb.20200825122502



Vancouver/ICMJE Style

Zawawy NAE, Soliman SI, Hantera NH. Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. Egypt. J. Exp. Biol. (Bot.). (2020), [cited November 03, 2020]; 16(2): 169-176. doi:10.5455/egyjebb.20200825122502



Harvard Style

Zawawy, N. A. E., Soliman, . S. I. & Hantera, . N. H. (2020) Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. Egypt. J. Exp. Biol. (Bot.), 16 (2), 169-176. doi:10.5455/egyjebb.20200825122502



Turabian Style

Zawawy, Nessma Ahmed El, Samira Ismail Soliman, and Nada Hani Hantera. 2020. Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 169-176. doi:10.5455/egyjebb.20200825122502



Chicago Style

Zawawy, Nessma Ahmed El, Samira Ismail Soliman, and Nada Hani Hantera. "Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 169-176. doi:10.5455/egyjebb.20200825122502



MLA (The Modern Language Association) Style

Zawawy, Nessma Ahmed El, Samira Ismail Soliman, and Nada Hani Hantera. "Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.2 (2020), 169-176. Print. doi:10.5455/egyjebb.20200825122502



APA (American Psychological Association) Style

Zawawy, N. A. E., Soliman, . S. I. & Hantera, . N. H. (2020) Vitis Vinifera L.: A new bioactive anticandidal agent to combat oral candidal carriage in Down syndrome patients.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 169-176. doi:10.5455/egyjebb.20200825122502