E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity

Hamed M. El-Shora, Mohammed H. Yassen, Reyad M. El-Sharkawy, Rehab A. Abdel Aziz.

Abstract
Laccase (EC 1:10: 3:2) is multicopper enzyme, belongs to the group of enzymes known as blue oxidases, which catalyzes the oxidation of a wide variety of organic compounds, polyphenols and aromatic amines. Laccase (AUMC 14100 gb, Accession No. MN219732) was purified from P. chrysogenum. The purification process included 85% ammonium sulphate precipitation, DEAE-Cellulose and Sephadex G-200. The specific activity and the fold of purification were 250 units mg-1 protein and 166.7-fold. The yield of purification was 57.6 from Sephadex G200 fraction. The results revealed that laccase stability at 60șC was enhanced in presence of sorbitol and mannitol as polyols was enhanced gradually in a concentration-dependent manner. Also, the enzyme stability in presence of raffinose was higher than that recorded with sucrose, maltose and xylose, respectively. This enhancement of the enzyme stability by such additives is critical results for industrial application of laccase.

Key words: Laccase, P. chrysogenum, Purification, Thermostability, Polyols, Raffinose.


 
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How to Cite this Article
Pubmed Style

El-Shora HM, Yassen MH, El-Sharkawy RM, Aziz RAA. Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. Egypt. J. Exp. Biol. (Bot.). 2021; 17(2): 167-172. doi:10.5455/egyjebb.20211117034103


Web Style

El-Shora HM, Yassen MH, El-Sharkawy RM, Aziz RAA. Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. http://www.egyseb.net//?mno=11615 [Access: December 28, 2021]. doi:10.5455/egyjebb.20211117034103


AMA (American Medical Association) Style

El-Shora HM, Yassen MH, El-Sharkawy RM, Aziz RAA. Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. Egypt. J. Exp. Biol. (Bot.). 2021; 17(2): 167-172. doi:10.5455/egyjebb.20211117034103



Vancouver/ICMJE Style

El-Shora HM, Yassen MH, El-Sharkawy RM, Aziz RAA. Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. Egypt. J. Exp. Biol. (Bot.). (2021), [cited December 28, 2021]; 17(2): 167-172. doi:10.5455/egyjebb.20211117034103



Harvard Style

El-Shora, H. M., Yassen, . M. H., El-Sharkawy, . R. M. & Aziz, . R. A. A. (2021) Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. Egypt. J. Exp. Biol. (Bot.), 17 (2), 167-172. doi:10.5455/egyjebb.20211117034103



Turabian Style

El-Shora, Hamed M., Mohammed H. Yassen, Reyad M. El-Sharkawy, and Rehab A. Abdel Aziz. 2021. Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (2), 167-172. doi:10.5455/egyjebb.20211117034103



Chicago Style

El-Shora, Hamed M., Mohammed H. Yassen, Reyad M. El-Sharkawy, and Rehab A. Abdel Aziz. "Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17 (2021), 167-172. doi:10.5455/egyjebb.20211117034103



MLA (The Modern Language Association) Style

El-Shora, Hamed M., Mohammed H. Yassen, Reyad M. El-Sharkawy, and Rehab A. Abdel Aziz. "Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 17.2 (2021), 167-172. Print. doi:10.5455/egyjebb.20211117034103



APA (American Psychological Association) Style

El-Shora, H. M., Yassen, . M. H., El-Sharkawy, . R. M. & Aziz, . R. A. A. (2021) Thermostability of Fungal Laccase and the Impact of Amino Acids on its Activity. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 17 (2), 167-172. doi:10.5455/egyjebb.20211117034103