E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers

Marwa Hamouda, Ashraf S. Haider, Eman Elbehairy, Adel R. Elshanshory.

Abstract
The role of genetic diversity is crucial for future improvements to meet societal demand for food security under a climate change scenario. Morphological, biochemical and molecular analyses have been suggested for the evaluation of genetic diversity in common bean (Phaseolus vulgaris L.) from Egypt. Twelve cultivars were collected from different five geographical isolated locations. These cultivars were evaluated using 20 morphological traits (15 qualitative and 5quantitative). The two cultivars P9 and P14 which were collected from Quesna and New Valley, respectively exhibited partially different morphologically features from other cultivars. The electrophoretic pattern of seed storage proteins indicated 45 different molecular weight bands, ranging from 309.7 kDa to 44.3 kDa with the presence of 4 unique bands and 41 polymorphic bands. ISSR results revealed a high level of polymorphism (69.1%) using six ISSR primers, which produced a total of 117 bands; out of them 85 bands are polymorphic, 25 monomorphic and 7 unique. Cluster analysis using CAP software program based on combined data revealed two groups. The obtained data confirmed that morphological, SDS-protein and ISSR markers are important tools for genetic analysis for common bean and recommended to give accurate results.

Key words: Common beans, Phaseolus vulgaris L., SDS-PAGE, ISSR


 
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How to Cite this Article
Pubmed Style

Hamouda M, Haider AS, Elbehairy E, Elshanshory AR. Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 129-139. doi:10.5455/egyjebb.20200721080720


Web Style

Hamouda M, Haider AS, Elbehairy E, Elshanshory AR. Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. http://www.egyseb.net//?mno=107581 [Access: September 25, 2020]. doi:10.5455/egyjebb.20200721080720


AMA (American Medical Association) Style

Hamouda M, Haider AS, Elbehairy E, Elshanshory AR. Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 129-139. doi:10.5455/egyjebb.20200721080720



Vancouver/ICMJE Style

Hamouda M, Haider AS, Elbehairy E, Elshanshory AR. Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. Egypt. J. Exp. Biol. (Bot.). (2020), [cited September 25, 2020]; 16(2): 129-139. doi:10.5455/egyjebb.20200721080720



Harvard Style

Hamouda, M., Haider, . A. S., Elbehairy, . E. & Elshanshory, . A. R. (2020) Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. Egypt. J. Exp. Biol. (Bot.), 16 (2), 129-139. doi:10.5455/egyjebb.20200721080720



Turabian Style

Hamouda, Marwa, Ashraf S. Haider, Eman Elbehairy, and Adel R. Elshanshory. 2020. Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 129-139. doi:10.5455/egyjebb.20200721080720



Chicago Style

Hamouda, Marwa, Ashraf S. Haider, Eman Elbehairy, and Adel R. Elshanshory. "Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 129-139. doi:10.5455/egyjebb.20200721080720



MLA (The Modern Language Association) Style

Hamouda, Marwa, Ashraf S. Haider, Eman Elbehairy, and Adel R. Elshanshory. "Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.2 (2020), 129-139. Print. doi:10.5455/egyjebb.20200721080720



APA (American Psychological Association) Style

Hamouda, M., Haider, . A. S., Elbehairy, . E. & Elshanshory, . A. R. (2020) Genetic variation among common beans cultivars (Phaseolus vulgaris L.) as revealed by morphological, protein and molecular markers. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 129-139. doi:10.5455/egyjebb.20200721080720