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2023, Vol: 19, Issue: 2
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Original Research 


Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions

Abd El-Raheem Ramadan El-Shanshoury , Nanis Gamal Allam, Eman Mohsen Basiouny , Maha Mahmoud Azab.

Abstract
Extracellular polymeric substances (EPSs) are ecologically friendly natural polymers produced by microorganisms in the surrounding media. Due to their varied chemical composition, exopolysaccharides are widely used in a variety of applications, including food, pharmaceuticals, and medical applications. In this work, thirty-six isolates of Actinobacteria isolated on starch nitrate medium from various locations of Egyptian soils were assessed for exopolysaccharide (EPS) synthesis. The isolated bacterium that produced the most EPS was Actinobacterium 10 (Streptomyces plicatus). Several culture and environmental parameters were investigated during the microbial growth in submerged culture to enhance bacterial growth and EPS production. The optimal crude EPS generation of 8.62 g/l was achieved after 7 days of incubation at 20 g/l starch concentration, at 35 °C and pH 7, under shaking at 120 rpm, with the most significant impacts. The findings of this study pave the door to produce large quantities of EPS from soil Actinobacteria for food and medicinal purposes.

Key words: Exopolysaccharides, Actinobacteria, Optimization, Culture conditions


 
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How to Cite this Article
Pubmed Style

AERE, Allam NG, EMB, Azab MM, . Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 151-160. doi:10.5455/egyjebb.20220525052945

Web Style

AERE, Allam NG, EMB, Azab MM, . Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. https://www.egyseb.net//?mno=47234 [Access: December 15, 2023]. doi:10.5455/egyjebb.20220525052945

AMA (American Medical Association) Style

AERE, Allam NG, EMB, Azab MM, . Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. Egypt. J. Exp. Biol. (Bot.). 2022; 18(1): 151-160. doi:10.5455/egyjebb.20220525052945


Vancouver/ICMJE Style

AERE, Allam NG, EMB, Azab MM, . Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. Egypt. J. Exp. Biol. (Bot.). (2022), [cited December 15, 2023]; 18(1): 151-160. doi:10.5455/egyjebb.20220525052945


Harvard Style

, A. E. R. E., Allam, N. G., , E. M. B., Azab, M. M. & (2022) Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. Egypt. J. Exp. Biol. (Bot.), 18 (1), 151-160. doi:10.5455/egyjebb.20220525052945


Turabian Style

, Abd El-Raheem Ramadan El-Shanshoury, Nanis Gamal Allam, Eman Mohsen Basiouny, Maha Mahmoud Azab, and . 2022. Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 151-160. doi:10.5455/egyjebb.20220525052945


Chicago Style

, Abd El-Raheem Ramadan El-Shanshoury, Nanis Gamal Allam, Eman Mohsen Basiouny, Maha Mahmoud Azab, and . "Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 151-160. doi:10.5455/egyjebb.20220525052945


MLA (The Modern Language Association) Style

, Abd El-Raheem Ramadan El-Shanshoury, Nanis Gamal Allam, Eman Mohsen Basiouny, Maha Mahmoud Azab, and . "Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.1 (2022), 151-160. Print. doi:10.5455/egyjebb.20220525052945


APA (American Psychological Association) Style

, A. E. R. E., Allam, N. G., , E. M. B., Azab, M. M. & (2022) Optimization of Exopolysaccharide Production by Soil Actinobacterium Streptomyces plicatus under Submerged Culture Conditions. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (1), 151-160. doi:10.5455/egyjebb.20220525052945


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