E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.

Fathi A. Mansour *Yehia A. El-Zawahry **Mohamed A. Abouelela Mohamed M. Khashaba.

Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a specific strain of the bacterium Staphylococcus aureus that has developed antibiotic resistance to all penicillins, including methicillin and other narrow-spectrum β-lactamase-resistant penicillin antibiotics. The purpose of this study is to investigate the presence methicillin resistant S. aureus strains in patients suffering from wound infections and septicemia, in Mansoura University Hospitals (MUHs). A total of 29 MRSA strains were isolated from nosocomially infected patients. All isolates were subjected to antibiogram comparison, polymerase chain reaction (PCR) assays for amplifying mecA, oxacillin agar screen test and latex agglutination test. PCR detected 18 out of 29 strains (62.1%), oxacillin agar detected 22 out of 29 strains (75.9%), while latex screen showed 25 out of 29 MRSA strains (86.2%); P<0.001. MRSA strains were completely resistant to Cefuroxime, Cephalexine and Cefotaxime, but appeared to be quite susceptible to vancomycin. This study has demonstrated detection of MRSA strains by different susceptibility procedures and PCR test as a golden standard method for MRSA identification. Our findings call for the prospective potentiality of oxacillin agar screen and latex agglutination tests for detection of MRSA.

Key words: methicillin resistance, mecA gene, PCR, Staphylococcus aureus, nosocomial infection, MRSA.


 
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Pubmed Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 59-65.

Web Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. https://www.egyseb.net//?mno=187197 [Access: December 07, 2023].

AMA (American Medical Association) Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 59-65.


Vancouver/ICMJE Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. Egypt. J. Exp. Biol. (Bot.). (2009), [cited December 07, 2023]; 5(0): 59-65.


Harvard Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba (2009) DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. Egypt. J. Exp. Biol. (Bot.), 5 (0), 59-65.


Turabian Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. 2009. DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 59-65.


Chicago Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. "DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5 (2009), 59-65.


MLA (The Modern Language Association) Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba. "DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5.0 (2009), 59-65. Print.


APA (American Psychological Association) Style

Fathi A. Mansour Yehia A. El-Zawahry Mohamed A. Abouelela Mohamed M. Khashaba (2009) DETECTION OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS RESPONSIBLE FOR WOUND INFECTIONS AND SEPTICEMIA IN MANSOURA UNIVERSITY HOSPITALS.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 59-65.


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