E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE

Sehand K. Arif Solaf M. Essa.

Abstract
Brucellosis is a zoonotic disease and its traditional diagnosis is based on blood culture and serological methods, for more sensitive and specific detection, PCR method is recommended. In the present study we targeted the Brucella genus-specific 16S rRNA for detection of Brucellosis using peripheral blood by nested-PCR technique. Fifty-five blood samples were gathered from suspected outpatients from Sulaimani province. Diagnosis was established depending on ELISA and Rose Bengal test and later was examined by PCR method. Brucella spp were detected in Fifty-two patients (1100 bp), while when we targeted the sequences found only in B. melitensis, fifty suspected patients showed a positive PCR product of 958 bp. Fifteen patients were male (27%) and 40 were female (73%), and their ages ranged from 5-65 yr old (mean, 36 yr). The highest number of Brucellosis (Considering ELISA and PCR) cases was found in the >30-40 years age group (20, 36%), while the lowest number was found in <10 years age group and >60 age group (2, 4%) for each. PCR technique is an easy and rapid assay for diagnosis of Brucellosis in suspected cases.

Key words: B. melitensis, PCR, 16S rRNA


 
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Pubmed Style

Sehand K. Arif Solaf M. Essa. RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 119-124.

Web Style

Sehand K. Arif Solaf M. Essa. RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. https://www.egyseb.net//?mno=186935 [Access: December 15, 2023].

AMA (American Medical Association) Style

Sehand K. Arif Solaf M. Essa. RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 119-124.


Vancouver/ICMJE Style

Sehand K. Arif Solaf M. Essa. RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. Egypt. J. Exp. Biol. (Bot.). (2011), [cited December 15, 2023]; 7(1): 119-124.


Harvard Style

Sehand K. Arif Solaf M. Essa (2011) RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. Egypt. J. Exp. Biol. (Bot.), 7 (1), 119-124.


Turabian Style

Sehand K. Arif Solaf M. Essa. 2011. RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 119-124.


Chicago Style

Sehand K. Arif Solaf M. Essa. "RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7 (2011), 119-124.


MLA (The Modern Language Association) Style

Sehand K. Arif Solaf M. Essa. "RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7.1 (2011), 119-124. Print.


APA (American Psychological Association) Style

Sehand K. Arif Solaf M. Essa (2011) RAPID DIAGNOSIS OF HUMAN BRUCELLOSIS FROM BLOOD SAMPLES USING POLYMERASE CHAIN REACTION TECHNIQUE. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 119-124.


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The Egyptian Journal of Experimental Biology (Botany) publishes original scientific research concerning botanical sciences (Morphology, Taxonomy, Gen ... Read more.



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