E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
19 / 2Current Issue Online FirstArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Original Research 


MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm.

Abstract
Lignin is a complex, aromatic polymer that limits plant cell wall degradation. Genetic Engineering using an antisense system offers the way to modulate enzymes in the lignin pathway and lead to reduce lignin. Caffeic acid 3-O-methyltransferase (COMT) catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and is believed to occupy a pivotal position in the lignin biosynthetic pathway. The plasmid pMAS-COMT 10.907bp has COMT antisense to down regulate O-methyltransferase and reduce lignin level in maize and bar gene as selectable marker that confers glufosinate herbicide resistance under 35s promoter. Out of fifty transgenic lines expressed bar gene and COMT-Antisense, only, three lines have significant differences from wild type. The results of this study pointed to possible use of genetic transformation approach to minimize lignin content and consequently enhancing bioethanol production from cellulosic material (stover).

Key words: Zea mays, COMT- antisense, gene bombardment, lignin content


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 59-66.

Web Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. https://www.egyseb.net//?mno=186929 [Access: December 05, 2023].

AMA (American Medical Association) Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 59-66.


Vancouver/ICMJE Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. Egypt. J. Exp. Biol. (Bot.). (2011), [cited December 05, 2023]; 7(1): 59-66.


Harvard Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm (2011) MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. Egypt. J. Exp. Biol. (Bot.), 7 (1), 59-66.


Turabian Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. 2011. MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 59-66.


Chicago Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. "MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7 (2011), 59-66.


MLA (The Modern Language Association) Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm. "MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7.1 (2011), 59-66. Print.


APA (American Psychological Association) Style

Manal A. Abdel-Rhman Ibrahim E. Mousa Kyoung Kim Jack M. Widholm (2011) MODIFICATION OF LIGNIN CONTENT IN MAIZE USING GENETIC TRANSFORMATION. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 59-66.


Author LoginReviewer LoginAbout PublisherEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicySelf-Archiving PoliciesTerms of UseLicense Information

About THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)

The Egyptian Journal of Experimental Biology (Botany) publishes original scientific research concerning botanical sciences (Morphology, Taxonomy, Gen ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

egysebbz@gmail.com