E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid.

Abstract
Future warming and changes in the climate have dramatic effects on natural resources and biodiversity of the desert. Plants in the desert are frequently subjected to abiotic stress that affects adversely their growth and development as well as canopy level. Drought and heat stresses are among the factors which are generally expected to have significant effects on genetic diversity of natural plant populations. In this study, random amplified polymorphic DNA (RAPD) technique was used to reveal the genetic diversity and assess differences among and between six Zilla spinosa (L.) populations grown in different habitats along Wadi Hagul during spring and summer seasons and to determine the geographical differences at the population level by examining molecular characteristics. Eight informative and reproducible primers were chosen for RAPD analysis. These random primers generated a total of 204 RAPD fragments including 199 polymorphic bands with 97.5% polymorphism. OPA-10, OPA-11, OPD-15, and OPC-03 primers gave very high ratios (100%) of polymorphic bands in PCR reactions. However, OPB-06, OPB-07, OPD-14 and OPC-01 displayed polymorphism between 92.6% and 96.9%. The lowest value of polymorphism (92.6%) was achieved by OPD-14 primer. The relationships between the genetic diversity indexes on one hand and the geographic and climatic factors on the other hand were estimated by the Pearson correlation with SPSS 11.0 software. The results of the correlation analysis show that there were significant (P<0.05) or highly significant (P<0.01) correlations between each of the genetic diversity indexes and the different temperature of spring and summer seasons and water scarcity which were mainly caused by the different populations habitats.

Key words: Zilla spinosa (L.), Environmental stress; Genetic diversity; Random amplified polymorphic DNA (RAPD)


 
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Pubmed Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. Egypt. J. Exp. Biol. (Bot.). 2014; 10(2): 115-124.

Web Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. https://www.egyseb.net//?mno=186621 [Access: December 11, 2023].

AMA (American Medical Association) Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. Egypt. J. Exp. Biol. (Bot.). 2014; 10(2): 115-124.


Vancouver/ICMJE Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. Egypt. J. Exp. Biol. (Bot.). (2014), [cited December 11, 2023]; 10(2): 115-124.


Harvard Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid (2014) SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. Egypt. J. Exp. Biol. (Bot.), 10 (2), 115-124.


Turabian Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. 2014. SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 10 (2), 115-124.


Chicago Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. "SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 10 (2014), 115-124.


MLA (The Modern Language Association) Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid. "SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 10.2 (2014), 115-124. Print.


APA (American Psychological Association) Style

Hemmat Khattab Ibrahim Khattab Hesham M. El-Shishtawy Hosny A. Musallam Amal A. Morsy Zeinab D. S. Almarid (2014) SEASONAL AND SPATIAL VARIATIONS IN THE GENETIC DIVERSITY OF ZILLA SPINOSA (L.) INHABITING WADI HAGUL AS REVEALED BY RAPD MARKERS. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 10 (2), 115-124.


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The Egyptian Journal of Experimental Biology (Botany) publishes original scientific research concerning botanical sciences (Morphology, Taxonomy, Gen ... Read more.



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