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2023, Vol: 19, Issue: 2
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Original Research 


Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil

Amr El-Waseif, Nevin Ibrahim, Nagwa Abdallah, Shimaa Ahmed.

Abstract
The present study focused on the antimicrobial compounds production ‎from Streptomyces clavuligerus isolated from Egyptian soil. The results ‎revealed that the isolate had a broad spectrum antimicrobial activity ‎against Escherichia coli NCTC 10416, Salmonella typhi NCIMB 9331, ‎Bacillus subtilis NRRL B-543, Staphylococcus aureus ATCC 29213 and ‎Candida albicans ATCC 10231. The isolate was identified according to ‎the morphological and molecular techniques identification as ‎Streptomyces clavuligerus. Different experiments were conducted to ‎study the optimum conditions for the highest antimicrobial productivity of ‎Streptomyces clavuligerus. Results indicated that, the optimum medium ‎for the production of antimicrobial compound(s) was YPSS- medium ‎included carbon source starch at concentration of 10 g/l and nitrogen ‎source urea at concentration of 6 g/l. In addition the optimum incubation ‎period was 7 days at incubation temperature 30oC if the optimum ‎agitation speed was 150 rpm and initial pH was 6.5 using phosphate ‎buffer. On the other hand, peel potato was the best carbon by product ‎source while whey was the best nitrogen by product source. The ‎extraction, purification and structure identification of the antimicrobial ‎agent(s) produced by Streptomyces clavuligerus, were carried out by ‎UVanalysis, IR and Mass spectrum which identified as Clavulanic acid ‎and Holomycin.‎

Key words: Streptomyces clavuligerus, Optimization conditions, Clavulanic acid and ‎Holomycin.‎


 
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How to Cite this Article
Pubmed Style

El-Waseif A, Ibrahim N, Abdallah N, Ahmed S, . Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. Egypt. J. Exp. Biol. (Bot.). 2018; 14(2): 353-383. doi:10.5455/egyjebb20181112035943

Web Style

El-Waseif A, Ibrahim N, Abdallah N, Ahmed S, . Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. https://www.egyseb.net//?mno=16842 [Access: December 06, 2023]. doi:10.5455/egyjebb20181112035943

AMA (American Medical Association) Style

El-Waseif A, Ibrahim N, Abdallah N, Ahmed S, . Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. Egypt. J. Exp. Biol. (Bot.). 2018; 14(2): 353-383. doi:10.5455/egyjebb20181112035943


Vancouver/ICMJE Style

El-Waseif A, Ibrahim N, Abdallah N, Ahmed S, . Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. Egypt. J. Exp. Biol. (Bot.). (2018), [cited December 06, 2023]; 14(2): 353-383. doi:10.5455/egyjebb20181112035943


Harvard Style

El-Waseif, A. . ., Ibrahim, N. ., Abdallah, N. ., Ahmed, S. . & (2018) Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. Egypt. J. Exp. Biol. (Bot.), 14 (2), 353-383. doi:10.5455/egyjebb20181112035943


Turabian Style

El-Waseif, Amr , Nevin Ibrahim, Nagwa Abdallah, Shimaa Ahmed, and . 2018. Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (2), 353-383. doi:10.5455/egyjebb20181112035943


Chicago Style

El-Waseif, Amr , Nevin Ibrahim, Nagwa Abdallah, Shimaa Ahmed, and . "Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14 (2018), 353-383. doi:10.5455/egyjebb20181112035943


MLA (The Modern Language Association) Style

El-Waseif, Amr , Nevin Ibrahim, Nagwa Abdallah, Shimaa Ahmed, and . "Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14.2 (2018), 353-383. Print. doi:10.5455/egyjebb20181112035943


APA (American Psychological Association) Style

El-Waseif, A. . ., Ibrahim, N. ., Abdallah, N. ., Ahmed, S. . & (2018) Biosynthesis, Optimization and Structure Elucidation of ‎Antimicrobial Agent Produced by Streptomyces clavuligerus ‎isolated from Egyptian soil. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (2), 353-383. doi:10.5455/egyjebb20181112035943


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