E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM

Aida M. Farag, Ahmed M. Mohammed, Magdy M. Afifi, Usama M. Abdel Raouf.

Abstract
Eighteen bacterial strains were isolated from Sea water collected from different sites of Red Sea, Egypt. They were screened for antagonistic activity against many bacterial pathogens. The most active antimicrobial producer (R6) which isolated from Safaga middle was identified as Bacillus badius strain AAUM using 16S rRNA phylogenetic analysis. The most control variables were selected from Plackett–Burman (PB) factorial design for the production of antimicrobial agent from Bacillus badius strain AAUM. It achieved 1.56-fold fold increase (against S. aureus) when grown in medium composed of g/l: peptone, 2.5; yeast extract, 5.0; sea water, 25ml; size of inoculum (3ml/50ml), adjusted to pH 9 and incubation period 20 hrs at 45oC. Immobilization using entrapment and adsorption techniques were applied to improve the productivity of cells. B. badius strain AAUM adsorbed on polyurethane foam realized 1.4-fold increase than the free cells. Reused of the adsorbed culture caused an increase of antimicrobial agent production by 1.14-fold than the free cells. The predicted crude antimicrobial spectra suggested that the B. badius strain AAUM can produce important and novel antimicrobial drugs.

Key words: Bacillus, Antimicrobial agents, Optimization, Plackett–Burman, immobilization


 
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How to Cite this Article
Pubmed Style

Farag AM, Mohammed AM, Afifi MM, Raouf UMA. Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 135-146. doi:10.5455/egyjebb.20190407015126


Web Style

Farag AM, Mohammed AM, Afifi MM, Raouf UMA. Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. http://www.egyseb.net/?mno=42132 [Access: October 20, 2019]. doi:10.5455/egyjebb.20190407015126


AMA (American Medical Association) Style

Farag AM, Mohammed AM, Afifi MM, Raouf UMA. Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 135-146. doi:10.5455/egyjebb.20190407015126



Vancouver/ICMJE Style

Farag AM, Mohammed AM, Afifi MM, Raouf UMA. Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. Egypt. J. Exp. Biol. (Bot.). (2019), [cited October 20, 2019]; 15(2): 135-146. doi:10.5455/egyjebb.20190407015126



Harvard Style

Farag, A. M., Mohammed, . A. M., Afifi, . M. M. & Raouf, . U. M. A. (2019) Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. Egypt. J. Exp. Biol. (Bot.), 15 (2), 135-146. doi:10.5455/egyjebb.20190407015126



Turabian Style

Farag, Aida M., Ahmed M. Mohammed, Magdy M. Afifi, and Usama M. Abdel Raouf. 2019. Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 135-146. doi:10.5455/egyjebb.20190407015126



Chicago Style

Farag, Aida M., Ahmed M. Mohammed, Magdy M. Afifi, and Usama M. Abdel Raouf. "Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 135-146. doi:10.5455/egyjebb.20190407015126



MLA (The Modern Language Association) Style

Farag, Aida M., Ahmed M. Mohammed, Magdy M. Afifi, and Usama M. Abdel Raouf. "Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 135-146. Print. doi:10.5455/egyjebb.20190407015126



APA (American Psychological Association) Style

Farag, A. M., Mohammed, . A. M., Afifi, . M. M. & Raouf, . U. M. A. (2019) Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 135-146. doi:10.5455/egyjebb.20190407015126