E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA

Shaimaa Amer, Doaa Darwish.

Abstract
The present study carried out in order to enhance the production of L-asparaginase by bacteria isolated from cultivated farms and saline water in Saudi Arabia and their abilities to produce L- asparaginase. Optimum conditions of the enzyme production were achieved.
A total of thirteen samples collected from some areas at Saudi Arabia were isolated using M9 agar medium. Only five out 13 isolates were selected and screened according to higher yield of enzyme productivity. The strains were identified according to their morphological and cultural characteristics, phylogenetic analysis as well as 16S rRNA Gene Amplification and Sequencing. Optimization of fermentation parameters for L-asparaginase production were achieved after three days incubation periods, incubation temperature ranging from 30-40 o C, pH 9.0 and less commonly 8.0. The enzyme production was enhanced by maltose (carbon source), peptone and ammonium sulphate (nitrogen sources) and divalent iron (F+2) as a source of metal ions

Key words: L-asparaginase, characterization, optimization, bacteria


 
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How to Cite this Article
Pubmed Style

Amer S, Darwish D. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228


Web Style

Amer S, Darwish D. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. http://www.egyseb.net/?mno=40729 [Access: August 19, 2019]. doi:10.5455/egyjebb.20190331043228


AMA (American Medical Association) Style

Amer S, Darwish D. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228



Vancouver/ICMJE Style

Amer S, Darwish D. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). (2019), [cited August 19, 2019]; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228



Harvard Style

Amer, S. & Darwish, . D. (2019) PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228



Turabian Style

Amer, Shaimaa, and Doaa Darwish. 2019. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228



Chicago Style

Amer, Shaimaa, and Doaa Darwish. "PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 227-234. doi:10.5455/egyjebb.20190331043228



MLA (The Modern Language Association) Style

Amer, Shaimaa, and Doaa Darwish. "PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 227-234. Print. doi:10.5455/egyjebb.20190331043228



APA (American Psychological Association) Style

Amer, S. & Darwish, . D. (2019) PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228