E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA

Shaimaa Amer, Doaa Darwish.

Abstract
The present study carried out in order to enhance the production of L-asparaginase by bacteria isolated from cultivated farms and saline water in Saudi Arabia and their abilities to produce L- asparaginase. Optimum conditions of the enzyme production were achieved.
A total of thirteen samples collected from some areas at Saudi Arabia were isolated using M9 agar medium. Only five out 13 isolates were selected and screened according to higher yield of enzyme productivity. The strains were identified according to their morphological and cultural characteristics, phylogenetic analysis as well as 16S rRNA Gene Amplification and Sequencing. Optimization of fermentation parameters for L-asparaginase production were achieved after three days incubation periods, incubation temperature ranging from 30-40 o C, pH 9.0 and less commonly 8.0. The enzyme production was enhanced by maltose (carbon source), peptone and ammonium sulphate (nitrogen sources) and divalent iron (F+2) as a source of metal ions

Key words: L-asparaginase, characterization, optimization, bacteria


 
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How to Cite this Article
Pubmed Style

Amer S, Darwish D, . PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228

Web Style

Amer S, Darwish D, . PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. https://www.egyseb.net//?mno=40729 [Access: December 05, 2023]. doi:10.5455/egyjebb.20190331043228

AMA (American Medical Association) Style

Amer S, Darwish D, . PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). 2019; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228


Vancouver/ICMJE Style

Amer S, Darwish D, . PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.). (2019), [cited December 05, 2023]; 15(2): 227-234. doi:10.5455/egyjebb.20190331043228


Harvard Style

Amer, S., Darwish, D. & (2019) PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. Egypt. J. Exp. Biol. (Bot.), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228


Turabian Style

Amer, Shaimaa, Doaa Darwish, and . 2019. PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228


Chicago Style

Amer, Shaimaa, Doaa Darwish, and . "PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15 (2019), 227-234. doi:10.5455/egyjebb.20190331043228


MLA (The Modern Language Association) Style

Amer, Shaimaa, Doaa Darwish, and . "PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 15.2 (2019), 227-234. Print. doi:10.5455/egyjebb.20190331043228


APA (American Psychological Association) Style

Amer, S., Darwish, D. & (2019) PRODUCTION AND OPIMIZATION OF L-ASPARAGINASE BY BACTERIA ISOLATED FROM SAUDI ARABIA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 15 (2), 227-234. doi:10.5455/egyjebb.20190331043228


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