E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188)

Nanis Gamal El-Deen Allam, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, Shaimaa Abdel Aziz Ahmed Abdel Kawy.

Abstract
Androstenedione (AD) and androstadienedione (ADD) are steroids intermediate valuable for the production of steroid medicaments. Microbial bioconversion of phytosterols to produce AD is a well researched area in synthesis of steroid hormones. The current study focused on bioconversion of β, sitosterol as a plant phytosterol into androstenedione (AD) and androstadienedione (ADD) optimized by Ochrobactrum anthropi strain (ATCC 49188). The study involved the effect of some physiological and biochemical factors affecting on the bioconversion process such as inoculums size, inoculum age, pH of the fermentation medium No.Ⅰ, (g/L) glucose 10 K2HPO4 0.75, KH2PO4 3, (NH4)2SO4 1, MgSO4 .7H2O 1, 8 hydroxyqunioline 0.8), temperature 30as well as substrate concentration. The results showed that the maximum AD (39.29%) output was obtained by using fermentation medium No.Ⅰ at pH 8; inoculum size 2ml/100 ml; 24h inoculum age, temperature 30and 15mg/100ml substrate concentration.

Key words: androstenedione (AD), androstadienedione (ADD), Phytosterols, Microbial bioconversion, Ochrobactrum anthropic


 
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How to Cite this Article
Pubmed Style

Allam NGE, Salam ISA, Shabana SA, Kawy SAAAA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939


Web Style

Allam NGE, Salam ISA, Shabana SA, Kawy SAAAA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). http://www.egyseb.net//?mno=18341 [Access: December 21, 2020]. doi:10.5455/egyjebb.20201202100939


AMA (American Medical Association) Style

Allam NGE, Salam ISA, Shabana SA, Kawy SAAAA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939



Vancouver/ICMJE Style

Allam NGE, Salam ISA, Shabana SA, Kawy SAAAA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939



Harvard Style

Allam, N. G. E., Salam, I. S. A., Shabana, S. A., Kawy, S. A. A. A. A. & (2020) Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939



Turabian Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, Shaimaa Abdel Aziz Ahmed Abdel Kawy, and . 2020. Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939



Chicago Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, Shaimaa Abdel Aziz Ahmed Abdel Kawy, and . "Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188)." doi:10.5455/egyjebb.20201202100939



MLA (The Modern Language Association) Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, Shaimaa Abdel Aziz Ahmed Abdel Kawy, and . "Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188)." doi:10.5455/egyjebb.20201202100939



APA (American Psychological Association) Style

Allam, N. G. E., Salam, I. S. A., Shabana, S. A., Kawy, S. A. A. A. A. & (2020) Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain (ATCC 49188). doi:10.5455/egyjebb.20201202100939