E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains

Hazem Hamed, Asaad Gaber, Mohammed Ali Ahmed Eid.

Abstract
Acinetobacter baumannii (A. baumannii) is suggested to be common cause infection and outbreaks. Carbapenems are often used as antibiotics of last resort for treating infections caused by A. baumannii. Metallo beta-lactamases (MBL) are liable for carbapenem resistance, as well as oxacillinases (OXA). A total of 70 non-repetitive imipenem and meropenem resistant Acinetobacter species were isolated from clinical samples. The VITEK-2 system was used for species identification and detection of antibiotic susceptibility. This was followed by modified Hodge test (MHT) and combined disc diffusion test (CDDT) for phenotypic detection of carbapenemase (also referred to OXA-like) and metallo-β-lactamase enzyme production, respectively. 92.8 % of the A. baumannii isolates were carbapenem-resistant, of which 100% were carbapenemase producers by MHT, and only 15.4% were metallo-β-lactamase producers by CDDT. PCR was used to detect carbapenemase genes (blaOXA-23, blaOXA-24, blaOXA- 51, and blaOXA-58). Our data showed that resistance to carbapenems is due to the presence of blaOXA-23, blaOXA-58 and blaOXA-24 genes. Resistance to imipenem was found to be a better indicator of MBL production. IPM-EDTA disk synergy test appears to be useful in differentiating MBL and non-metalloenzyme producers. During this study, we reported a reasonable detection tests of MBL and OXA producing species as a crucial step for an efficient infection control policy and may to prevent their uncontrolled spread in clinical settings.

Key words: Acinetobacter baumannii, Antibiotic resistance, Carbapenem resistance, Modified Hodge Test, Combined Disc Diffusion test.


 
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How to Cite this Article
Pubmed Style

Hamed H, Gaber A, Eid MAA. Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 223-230. doi:10.5455/egyjebb.20201012105100


Web Style

Hamed H, Gaber A, Eid MAA. Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. http://www.egyseb.net//?mno=12371 [Access: December 01, 2020]. doi:10.5455/egyjebb.20201012105100


AMA (American Medical Association) Style

Hamed H, Gaber A, Eid MAA. Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 223-230. doi:10.5455/egyjebb.20201012105100



Vancouver/ICMJE Style

Hamed H, Gaber A, Eid MAA. Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. Egypt. J. Exp. Biol. (Bot.). (2020), [cited December 01, 2020]; 16(2): 223-230. doi:10.5455/egyjebb.20201012105100



Harvard Style

Hamed, H., Gaber, . A. & Eid, . M. A. A. (2020) Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. Egypt. J. Exp. Biol. (Bot.), 16 (2), 223-230. doi:10.5455/egyjebb.20201012105100



Turabian Style

Hamed, Hazem, Asaad Gaber, and Mohammed Ali Ahmed Eid. 2020. Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 223-230. doi:10.5455/egyjebb.20201012105100



Chicago Style

Hamed, Hazem, Asaad Gaber, and Mohammed Ali Ahmed Eid. "Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 223-230. doi:10.5455/egyjebb.20201012105100



MLA (The Modern Language Association) Style

Hamed, Hazem, Asaad Gaber, and Mohammed Ali Ahmed Eid. "Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.2 (2020), 223-230. Print. doi:10.5455/egyjebb.20201012105100



APA (American Psychological Association) Style

Hamed, H., Gaber, . A. & Eid, . M. A. A. (2020) Reasonable Detection Tests of Metallo Beta-Lactamases and Oxacillinases Products in Carbapenem-Resistant Acinetobacter Strains. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 223-230. doi:10.5455/egyjebb.20201012105100